C4.zip May 2026

: The protein sample is aspirated through the tip, binding to the C4 resin.

: Best for proteins > 10 kDa and highly hydrophobic peptides.

: Contaminants are washed away with a dilute acid, such as trifluoroacetic acid (TFA) . c4.zip

Expression, Purification and Characterization of the Human ... - Nature

The C4 ZipTip is most commonly used in the workflow for (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight). : The protein sample is aspirated through the

This technology has been instrumental in recent studies, including identifying SARS-CoV-2 protease cleavage sites and characterizing human cannabinoid receptors.

A C4 ZipTip is a standard pipette tip containing a microscopic bed of resin fixed at its distal end. Unlike C18 resins, which are used for smaller peptides, the C4 resin is optimized for larger, more hydrophobic molecules like proteins and large polypeptides. Primary Uses in Research Researchers use these tools for two main reasons: Expression, Purification and Characterization of the Human

: Enrichment of dilute samples to ensure they are within the detection range of highly sensitive instruments. Role in Mass Spectrometry