: Beyond fluorescence, IFC provides data on cell size, shape, circularity, and internal complexity.
: If internal markers (e.g., nuclear proteins) are the target, cells are fixed with formaldehyde and permeabilized with detergents like Triton X-100. Imaging Flow Cytometry: Methods and Protocols
: Non-specific binding is prevented using anti-Fc antibodies. Cells are then stained with fluorophore-conjugated antibodies, viability dyes, or nuclear dyes like DAPI or Hoechst to provide spatial landmarks. : Beyond fluorescence, IFC provides data on cell
: Cells must be in a single-cell suspension to avoid clogging the thin fluidic channels used for imaging. : Beyond fluorescence